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从尿囊液中生产高滴度重组新城疫病毒
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Production of High-Titer Recombinant Newcastle Disease Virus from Allantoic Fluid
DOI:

07:24 min

May 25, 2022

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Chapters

  • 00:04Introduction
  • 00:55Purification of NDV from Allantoic Fluid
  • 05:08Quality Control Assays
  • 05:55Results: Production of High-Titer Recombinant NDV
  • 06:53Conclusion

Summary

Automatic Translation

在这里,我们提供了生产,纯化和定量高滴度重组新城疫病毒的详细程序。该方案始终产生>6×109 个斑块形成单位/ mL,提供适合体内动物研究 病毒量。描述了确保 体内 安全性的其他质量控制测定。

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