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从尿囊液中生产高滴度重组新城疫病毒
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JoVE Journal
Cancer Research
Production of High-Titer Recombinant Newcastle Disease Virus from Allantoic Fluid
Please note that all translations are automatically generated.
Click here for the English version.
从尿囊液中生产高滴度重组新城疫病毒
DOI:
10.3791/63817-v
•
07:24 min
•
May 25, 2022
•
Jacob G. E. Yates
,
Alexander Leacy
,
Phuc H. Pham
,
Nicole Zielinska
,
Emily A. Tusnadi
,
Leonardo Susta
,
Sarah K. Wootton
1
Department of Pathobiology
,
University of Guelph
Chapters
00:04
Introduction
00:55
Purification of NDV from Allantoic Fluid
05:08
Quality Control Assays
05:55
Results: Production of High-Titer Recombinant NDV
06:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们提供了生产,纯化和定量高滴度重组新城疫病毒的详细程序。该方案始终产生>6×10
9
个斑块形成单位/ mL,提供适合体内动物研究
的
病毒量。描述了确保
体内
安全性的其他质量控制测定。
Tags
Newcastle Disease Virus
Recombinant Virus
Virus Purification
Tangential Flow Filtration
Allantoic Fluid
Depth Filtration
Buffer Exchange
Pressure Control
Virus Concentration
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