Journal
/
/
Bottom-Up In Vitro Methods to assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
DOI:

09:09 min

August 17, 2022

, , , , , , , , , , ,

Chapters

  • 00:05Introduction
  • 00:49Designing Wavy NOA (Norland Optical Adhesive) Replica from Wavy PDMS (Polydimethylsiloxane) Patterns
  • 01:49Generation of a Supported Lipid Bilayer and Protein Incubation
  • 03:22Sample Fixation, Sample Dehydration, Critical Point Drying
  • 04:43Sample Mounting and Coating
  • 05:54Acquire the Images Using a Field-Emission SEM (FE-SEM) Microscope
  • 06:56Results: Analysis of the Self-Assembly of Septins at the Lipid Membrane
  • 08:27Conclusion

Summary

Automatic Translation

셉틴은 세포골격 단백질이다. 그들은 지질 막과 상호 작용하고 미크론 규모에서 막 곡률을 감지 할 수 있습니다. 우리는 막 변형, 곡률에 민감한 셉틴 결합 및 셉틴 필라멘트 초 구조를 분석하기위한 상향식 시험관 내 방법론을 설명합니다.

Related Videos

Read Article