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使用张力测量小体积腔肌图测量小鼠胸主动脉内皮依赖性血管松弛
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JoVE Journal
Biology
Measurement of Endothelium-Dependent Vasorelaxation in the Mouse Thoracic Aorta Using Tensometric Small Volume Chamber Myography
Please note that all translations are automatically generated.
Click here for the English version.
使用张力测量小体积腔肌图测量小鼠胸主动脉内皮依赖性血管松弛
DOI:
10.3791/63918-v
•
09:47 min
•
August 12, 2022
•
Brikena Gusek
,
Robert Folk
,
Tala Curry
,
Mitra Esfandiarei
1
Department of Biomedical Sciences, College of Graduate Studies
,
Midwestern University
,
2
Department of Child Health, College of Medicine-Phoenix
,
University of Arizona, Phoenix
Chapters
00:05
Introduction
00:56
Myograph Unit Preparation
02:03
Isolation and Mounting of the Aortic Segments onto the Myograph Chamber
03:24
Measurement of Endothelium-Dependent Vasorelaxation in Aortic Rings
05:18
Effects of General Inhibitors of NO Production on Endothelium-Mediated Vasorelaxation
06:25
Contribution of the Endothelial Layer to Aortic Vasorelaxation
08:00
Results: Mechanisms Underlying Endothelial-Dependent Vasorelaxation Using Tensometric Small Volume Chamber Myography in a Murine Model
09:06
Conclusion
Summary
Automatic Translation
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Automatic Translation
本协议描述了使用多腔肌图系统在小鼠主动脉内皮功能的实验
离体
评估中的张力肌图技术的概念和技术应用。
Tags
Tensometric Myography
Endothelium-dependent Vasorelaxation
Mouse Thoracic Aorta
HEPES-PSS Solution
High-potassium Solution
Carbogen Gas
Aortic Dissection
Aortic Segments
Myograph Unit
Data Acquisition
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