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RNA Fluorescence in situ Hybridization (FISH) to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
RNA Fluorescence in situ Hybridization (FISH) to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines

RNA Fluorescence in situ Hybridization (FISH) to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines

DOI:
10.3791/63980-v

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08:58 min

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July 27, 2022

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, , ,
1Department of Biology,San Diego State University, 2School of Biological Sciences,University of California, San Diego

Chapters

  • 00:05Introduction
  • 00:41FISH Staining with PFA Fixation
  • 06:15Results: FISH Staining of Wild C. tropicalis JU1848 Strain and C. elegans
  • 08:14Conclusion

Summary

Automatic Translation

Automatic Translation

Intestinal microbes, including extracellular bacteria and intracellular pathogens like the Orsay virus and microsporidia (fungi), are often associated with wild Caenorhabditis nematodes. This article presents a protocol for detecting and quantifying microbes that colonize and/or infect C. elegans nematodes, and for measuring pathogen load after controlled infections in the lab.

Tags

Keywords: RNA FISHMicrobial ColonizationCaenorhabditis ElegansIntestinesMicrobiome BacteriaIntracellular PathogensVisualizationIdentificationQuantificationFixationHybridization BufferFISH Probe

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