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Generering af multicue cellulære mikromiljøer ved UV-fotopatterning af tredimensionelle cellekultursubstrater
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates
DOI:

09:30 min

June 02, 2022

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Chapters

  • 00:05Introduction
  • 00:49Fabrication of 3D Cell Culture Substrates
  • 02:26Substrate Passivation of 3D Cell Culture Substrates
  • 04:01UV-Photopatterning of 3D Cell Culture Substrates
  • 05:27Protein Solution Preparation and Incubation
  • 06:18Cell Seeding and Image Acquisition
  • 07:16Results: Analysis of Human Dermal Fibroblasts and Human Primary Keratocytes on Patterned Surfaces
  • 08:51Conclusion

Summary

Automatic Translation

Traditionelt udføres cellekultur på plane substrater, der dårligt efterligner cellernes naturlige miljø in vivo. Her beskriver vi en metode til at producere cellekultursubstrater med fysiologisk relevante buede geometrier og mikropatternede ekstracellulære proteiner, hvilket muliggør systematiske undersøgelser af cellulær sensing af disse ekstracellulære signaler.

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