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Chapters
- 00:05Introduction
- 01:28Immunostaining of F-Actin and Activated-PAK1 for the Characterization of Tunneling Nanotubes
- 04:16Imaging with Confocal Microscopy
- 06:00Analysis of Confocal Z-Stack Images to Identify and Quantify Tunneling Nanotubes
- 09:453D Reconstruction of Z-Stack Images to Characterize Tunneling Nanotubes
- 11:00Results: Characterization of Tunneling Nanotubes by Constructing a 3D Volume View of Confocal Z Stack Images
- 12:11Conclusion
Tunneling nanorør (TNT'er) er primært åbne F-actin membran nanorør, der forbinder naboceller, hvilket letter intercellulær kommunikation. Den bemærkelsesværdige egenskab, der adskiller TNT'er fra andre cellefremspring, er nanorørenes svævende natur mellem celler. Her karakteriserer vi TNT'er ved at konstruere en 3D-volumenvisning af konfokale z-stack-billeder.
