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Anestesi og intubasjon av preadolescent musevalper for kardiotorakisk kirurgi
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JoVE Journal Biology
Anesthesia and Intubation of Preadolescent Mouse Pups for Cardiothoracic Surgery

Anestesi og intubasjon av preadolescent musevalper for kardiotorakisk kirurgi

DOI:
10.3791/64004-v

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09:47 min

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June 02, 2022

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, , , , ,
1Molecular Cardiology and Biophysics Division,Victor Chang Cardiac Research Institute, 2St Vincent’s Clinical School,University of New South Wales Sydney, 3Department of Anaesthesia,The Children’s Hospital at Westmead, 4School of Child and Adolescent Health,University of Sydney

Chapters

  • 00:05Introduction
  • 01:00Preparation of Instruments and Intubation of 10-Day-Old Mice
  • 03:09Myocardial Infarction Surgery of 10-Day-Old Mice
  • 05:13Post-Surgery Assessment of Infarct Size
  • 07:04Results: Anesthesia Regimen, Number of Intubation Attempts, and Post-Procedure Survival of 10-Day-Old Mouse Pups
  • 08:58Conclusion

Summary

Automatic Translation

Automatic Translation

Kardiotorakiske kirurgiske modeller hos mus >7 dager gamle krever intubasjon, men dette er utfordrende for preadolescent (8-14 dager gamle) musevalper, og det er lite informasjon om bedøvelsesregimer for intubasjon. Her presenterer vi doseringsregimer av ketamin/xylazin/atropin hos 10 dager gamle C57BL/6J musevalper som tillater endotrakeal intubasjon, samtidig som dyredødeligheten minimeres.

Tags

AnesthesiaIntubationPreadolescentMousePupsCardiothoracic SurgeryEndotrachealC57 Black Six J10-day OldCannulaLaryngoscopeTrachealVentilatorSpontaneous Breathing

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