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A Microfluidic Approach for the Study of Ice and Clathrate Hydrate Crystallization
JoVE Journal
Chemistry
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JoVE Journal Chemistry
A Microfluidic Approach for the Study of Ice and Clathrate Hydrate Crystallization

A Microfluidic Approach for the Study of Ice and Clathrate Hydrate Crystallization

DOI:
10.3791/64072-v

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08:01 min

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August 18, 2022

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,
1Department of Chemistry and Biochemistry,Yeshiva University, 2Department of Physics, Katz School of Science and Health,Yeshiva University

Chapters

  • 00:04Introduction
  • 00:37Microfluidic Device Fabrication
  • 02:45Setting up the Microfluidic Device and Formation of Single Crystals in the Microfluidic Channels
  • 04:23Solution Exchange Around Single Crystals
  • 04:47Experiments with Clathrate Hydrates
  • 05:59Results: Crystallization of Microscopic Ice Crystals and Clathrate Hydrates in Microfluidic Devices
  • 07:10Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes the crystallization of microscopic ice crystals and clathrate hydrates in microfluidic devices, enabling liquid exchange around the formed crystals. This provides unparalleled possibilities to examine the crystallization process and binding mechanisms of the inhibitors.

Tags

Keywords: MicrofluidicIce CrystallizationClathrate Hydrate CrystallizationAntifreeze ProteinsCrystal GrowthPDMSPlasma CleaningCold StageSapphire Disc

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