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Viral Transgene Expression in Rodent Hearts and the Assessment of Cardiac Arrhythmia Risk
 

Viral Transgene Expression in Rodent Hearts and the Assessment of Cardiac Arrhythmia Risk

Article DOI: 10.3791/64073-v • 05:15 min • July 27th, 2022
July 27th, 2022 •
1,2, 1,2, 1,2, 1, 1,2, 1,3, 1,2, 1, 1,2
1University of Ottawa Heart Institute, 2Department of Cellular and Molecular Medicine, University of Ottawa, 3Department of Biochemistry, Microbiology and Immunology, University of Ottawa

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Summary
Automatic Translation

The present protocol describes methods for transgene expression in rat and mouse hearts by direct intramyocardial injection of the virus under echocardiography guidance. Methods for the assessment of the susceptibility of hearts to ventricular arrhythmias by the programmed electrical stimulation of isolated, Langendorff-perfused hearts are also explained here.

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Keywords: Viral Transgene Expression Ventricular Myocardium Cardiac Arrhythmia Langendorff Perfusion ECG Isoproterenol Programmed Electrical Stimulation Ventricular Tachyarrhythmia
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