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Chapters
- 00:04Introduction
- 01:04Culturing HRMECs
- 03:46TEER Measurements
- 04:44Clathrin-Mediated Transcytosis Assay Using Cy3-Transferrin
- 06:00Caveolae-Mediated Transcytosis Assay Using Horse Radish Peroxidase (HRP)
- 07:29Results: Visualization of Endothelial Cell Transcytosis in the Retina and In Vitro Transcytosis Assay as a Model of BRB
- 10:14Conclusion
Denne protokol illustrerer et in vitro endotelcelletranscytoseassay som en model til evaluering af indre blod-retinal barrierepermeabilitet ved at måle evnen hos humane retinale mikrovaskulære endotelceller til at transportere peberrodsperoxidase på tværs af celler i caveolae-medierede transcellulære transportprocesser.
