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Eksperimentel melanom immunterapi model ved hjælp af tumorvaccination med et hæmatopoietisk cytokin
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Chapters
- 00:04Introduction
- 00:56Preparation of Cultured Tumor Cells for Implantation
- 02:33Tumor Implantation
- 03:19Vaccine Preparation and Injection of Flt3L-Expressing B16 F10 (B16-Flt3) Cells
- 04:20Intratumoral Immune Cell Isolation
- 06:59Results: Using a Cell-Based Tumor Vaccine Expressing Flt3 Model to Study Intratumoral Immune Cell Infiltrates
- 08:45Conclusion
Protokollen præsenterer en kræftimmunterapimodel ved hjælp af cellebaseret tumorvaccination med Flt3L-ekspressive B16-F10 melanom. Denne protokol demonstrerer procedurerne, herunder forberedelse af dyrkede tumorceller, tumorimplantation, cellebestråling, måling af tumorvækst, isolering af intratumorale immunceller og flowcytometrianalyse.
