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Experimentell melanomimmunterapimodell med tumörvaccination med ett hematopoetiskt cytokin
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Chapters
- 00:04Introduction
- 00:56Preparation of Cultured Tumor Cells for Implantation
- 02:33Tumor Implantation
- 03:19Vaccine Preparation and Injection of Flt3L-Expressing B16 F10 (B16-Flt3) Cells
- 04:20Intratumoral Immune Cell Isolation
- 06:59Results: Using a Cell-Based Tumor Vaccine Expressing Flt3 Model to Study Intratumoral Immune Cell Infiltrates
- 08:45Conclusion
Protokollet presenterar en cancerimmunterapimodell med cellbaserad tumörvaccination med Flt3L-uttryckande B16-F10-melanom. Detta protokoll visar procedurerna, inklusive beredning av odlade tumörceller, tumörimplantation, cellbestrålning, mätning av tumörtillväxt, isolering av intratumorala immunceller och flödescytometrianalys.
