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Journal
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Biochemistry
/
在膜上重构隔膜组件以研究生物物理性质和功能
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Biochemistry
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JoVE Journal
Biochemistry
Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions
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在膜上重构隔膜组件以研究生物物理性质和功能
DOI:
10.3791/64090-v
•
06:32 min
•
July 28, 2022
•
Brandy N. Curtis*
1
,
Ellysa J. D. Vogt*
2
,
Kevin S. Cannon
3
,
Amy S. Gladfelter
2,3
1
Department of Biochemistry and Biophysics
,
University of North Carolina at Chapel Hill
,
2
Curriculum in Genetics and Molecular Biology
,
University of North Carolina at Chapel Hill
,
3
Department of Biology
,
University of North Carolina at Chapel Hill
Chapters
00:05
Introduction
00:42
Planar Lipid Bilayers
02:46
Spherical Supported Lipid Bilayers
04:50
Results: Micrographs of Planar Bilayers and Lipid-Coated Microspheres
06:00
Conclusion
Summary
Automatic Translation
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Automatic Translation
无细胞重建一直是了解细胞骨架组装的关键工具,并且在过去十年中的工作已经建立了研究最小系统中隔膜蛋白动力学的方法。这里介绍的是三种在不同膜环境中观察隔膜组装的互补方法:平面双层,球形支撑和棒状支撑。
Tags
Keywords: Membrane Binding
Membrane Shape
Protein Assembly
Lipid Bilayer
Liposomes
Plasma Cleaning
UV-activated Adhesive
Supported Lipid Bilayer
Septins
TIRF Microscopy
Silica Microspheres
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