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在膜上重构隔膜组件以研究生物物理性质和功能
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions
DOI:

06:32 min

July 28, 2022

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Chapters

  • 00:05Introduction
  • 00:42Planar Lipid Bilayers
  • 02:46Spherical Supported Lipid Bilayers
  • 04:50Results: Micrographs of Planar Bilayers and Lipid-Coated Microspheres
  • 06:00Conclusion

Summary

Automatic Translation

无细胞重建一直是了解细胞骨架组装的关键工具,并且在过去十年中的工作已经建立了研究最小系统中隔膜蛋白动力学的方法。这里介绍的是三种在不同膜环境中观察隔膜组装的互补方法:平面双层,球形支撑和棒状支撑。

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