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Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy
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Neuroscience
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JoVE Journal Neuroscience
Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy

Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy

DOI:
10.3791/64096-v

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08:49 min

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August 01, 2022

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, , , , , , , , , , ,
1UNC Neuroscience Center,University of North Carolina, Chapel Hill, 2Department of Genetics,University of North Carolina, Chapel Hill, 3Department of Psychiatry,University of North Carolina, Chapel Hill, 4Center for Animal Magnetic Resonance Imaging,The University of North Carolina at Chapel Hill, 5Biomedical Research Imaging Center,The University of North Carolina at Chapel Hill, 6Department of Neurology,The University of North Carolina at Chapel Hill, 7Department of Computer Science,The University of North Carolina at Greensboro

Chapters

  • 00:05Introduction
  • 00:48Light-Sheet Imaging
  • 02:25Image Processing Using NuMorph
  • 04:12Image Analysis
  • 06:26Results: Quantification of Nuclear Markers Within Mouse Brain After Imaging on a Light-Sheet Microscope Using NuMorph
  • 07:57Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes methods for conducting magnetic resonance imaging, clearing, and immunolabeling of intact mouse brains using iDISCO+, followed by a detailed description of imaging using light-sheet microscopy, and downstream analyses using NuMorph.

Tags

Keywords: Whole-brainSingle-cellImagingAnalysisIntactNeonatalMouseBrainMRILight-sheet MicroscopyImage AnalysisNuclei QuantificationComputational PipelinePreprocessingCell DetectionImaging TimeComputational ResourcesSample MountingSample OrientationZoom MagnificationLight SheetNumerical ApertureDynamic FocusingLaser PowerLight Sheet WidthTile OverlapCondaNuMorphMATLABImage ProcessingIntensity Adjustment

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