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Fiksering af embryonalt musevæv til cytonemanalyse
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

Fiksering af embryonalt musevæv til cytonemanalyse

DOI:
10.3791/64100-v

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08:46 min

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June 16, 2022

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, , , ,
1Department of Cell and Molecular Biology,St. Jude Children’s Research Hospital, 2St. Jude Graduate School of Biomedical Sciences

Chapters

  • 00:04Introduction
  • 00:47Embryo Isolation and Whole Mount Staining
  • 03:12Embryo Embedding, Sectioning, and Mounting
  • 06:30Results: Visualization of Membrane Extensions in Tissue Sections
  • 08:13Conclusion

Summary

Automatic Translation

Automatic Translation

Her leveres en optimeret trin-for-trin protokol til fiksering, immunostaining og sektionering af embryoner for at detektere specialiseret signalfilopodia kaldet cytonomer i udvikling af musevæv.

Tags

Cytoneme AnalysisFixationLight MicroscopyEndogenous Signaling ProteinsPeritoneal CavityUterusYolk SacPlacentaParaformaldehydeHBSSPBSTritonBlocking SolutionPrimary AntibodySecondary AntibodyLow Melting Point Agarose

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