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Journal
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Developmental Biology
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シトネム解析のための胚性マウス組織の固定
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Fixation of Embryonic Mouse Tissue for Cytoneme Analysis
Please note that all translations are automatically generated.
Click here for the English version.
シトネム解析のための胚性マウス組織の固定
DOI:
10.3791/64100-v
•
08:46 min
•
June 16, 2022
•
Eric T. Hall
,
Christina A. Daly
2
,
Yan Zhang
,
Miriam E. Dillard
,
Stacey K. Ogden
1
Department of Cell and Molecular Biology
,
St. Jude Children’s Research Hospital
,
2
St. Jude Graduate School of Biomedical Sciences
Chapters
00:04
Introduction
00:47
Embryo Isolation and Whole Mount Staining
03:12
Embryo Embedding, Sectioning, and Mounting
06:30
Results: Visualization of Membrane Extensions in Tissue Sections
08:13
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、胚の固定、免疫染色、および切片化のために最適化された段階的なプロトコルが提供され、発達中のマウス組織におけるサイトネムと呼ばれる特殊なシグナル伝達糸状体を検出します。
Tags
Cytoneme Analysis
Fixation
Light Microscopy
Endogenous Signaling Proteins
Peritoneal Cavity
Uterus
Yolk Sac
Placenta
Paraformaldehyde
HBSS
PBS
Triton
Blocking Solution
Primary Antibody
Secondary Antibody
Low Melting Point Agarose
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