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Chapters
- 00:04Introduction
- 00:55Spinal Cord Tissue Dissection
- 01:34Magnetic Activated Cell Sorting (MACS)
- 02:39Seeding of Astrocytes for Reprogramming
- 04:25Reprogramming of Astrocytes (7‐Day Analysis)
- 05:29Results: Murine Astrocyte Cultures to Study Direct Neuronal Reprogramming
- 06:37Conclusion
Her beskriver vi en detaljeret protokol til at generere højt berigede kulturer af astrocytter afledt af forskellige regioner i centralnervesystemet hos postnatale mus og deres direkte omdannelse til funktionelle neuroner ved tvungen ekspression af transkriptionsfaktorer.
