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从原代细胞和培养细胞制备细胞质和核长RNA

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Cancer Research

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JoVE Journal Cancer Research

Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells

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从原代细胞和培养细胞制备细胞质和核长RNA

DOI:

10.3791/64199-v

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06:29 min

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April 07, 2023

•

Jacob Jahn, Sana Chaudhry, Maurizio Affer, Alejandro Pardo, Gabriel Pardo, Justin Taylor

¹Sylvester Comprehensive Cancer Center,University of Miami Miller School of Medicine

Chapters

00:04Introduction
01:11Separation of Nuclear and Cytoplasmic Fractions
01:56Validation of Nuclear-Cytoplasmic Separation
04:35Results: Confirmation of RNA Purity of Cytoplasmic and Nuclear Fraction
05:48Conclusion

Summary

Automatic Translation

本协议提供了一种高效灵活的方法，使用培养的细胞从核和细胞质级分中分离RNA，然后使用qPCR进行验证。这有效地替代了其他RNA制备试剂盒。

Tags

Keywords: Cytoplasmic RNA Nuclear RNA Cell Fractionation Lysis Buffer QPCR MALAT1 TUG1 Cell Culture Intracellular Interactions Nuclear-cytoplasmic Exchange Cancer Development

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