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Undersøgelse af mikrobielt samarbejde via billeddannelse massespektrometrianalyse af bakteriekolonier dyrket på agar og i væv under infektion
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Biochemistry
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JoVE Journal Biochemistry
Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Undersøgelse af mikrobielt samarbejde via billeddannelse massespektrometrianalyse af bakteriekolonier dyrket på agar og i væv under infektion

DOI:
10.3791/64200-v

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09:49 min

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November 18, 2022

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, , , ,
1Department of Chemistry,University of Florida, 2Division of Protective Immunity,Children’s Hospital of Philadelphia, 3Department of Pathology and Laboratory Medicine, Perelman School of Medicine,University of Pennsylvania

Chapters

  • 00:05Introduction
  • 00:58Ambient, Vacuum, and Heat-Mediated Drying of Clostridioides difficile and Enterococcus faecalis Bacterial Macrocolonies
  • 02:33MALDI Matrix Application via Robotic Spraying
  • 03:12Preparing Bacterial Macrocolonies for MALDI Imaging Mass Spectrometry Data Acquisition
  • 04:37Imaging Mass Spectrometry Data Analysis and Compound Identification
  • 05:57Cryosectioning of Noninfected Control Versus Clostridioides difficile-Infected Mouse Cecal Tissues
  • 07:45Preparation of Noninfected Control Versus Clostridioides difficile-Infected Mouse Cecal Tissues for Matrix Application and MALDI Imaging Mass Spectrometry
  • 08:08Results: Imaging Mass Spectrometry Analysis of Bacterial Macrocolonies and Infected Murine Cecal Tissues
  • 09:09Conclusion

Summary

Automatic Translation

Automatic Translation

En ny prøveforberedelsesmetode demonstreres til analyse af agarbaserede, bakterielle makrokolonier via matrixassisteret laserdesorptions-/ioniseringsbilleddannelsesmassespektrometri.

Tags

Keywords: Microbial CooperationImaging Mass SpectrometryBacterial ColoniesAgarMALDI MatrixSample PreparationVacuum DryingSpatial MetabolismMetabolitesMicrobial Pathogens

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