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Separation av immuncellssubpopulationer i perifera blodprover från barn med infektiös mononukleos
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1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital,Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children, 2019RU016,Chinese Academy of Medical Sciences
Chapters
- 00:05Introduction
- 00:36Isolation of CD14+ Monocytes from PBMCs Using CD14 Microbeads
- 02:38Fluorescently Labeled Antibody Staining
- 03:52Flow Cytometry Parameter Setting
- 04:32Gating
- 06:18Cell Sorting and Collection via Flow Cytometry
- 07:00Results: Comparison of Immune Cell Subpopulations from Patients with Infectious Mononucleosis, Healthy Epstein Barr Virus Carriers, and Uninfected Samples
- 08:14Conclusion
Vi beskriver en metod som kombinerar immunomagnetiska pärlor och fluorescensaktiverad cellsortering för att isolera och analysera definierade immuncellssubpopulationer av mononukleära celler i perifert blod (monocyter, CD4 + T-celler, CD8 + T-celler, B-celler och naturliga mördarceller). Med hjälp av denna metod kan magnetiska och fluorescerande märkta celler renas och analyseras.
