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A “Plug-And-Display” Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
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Bioengineering
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JoVE Journal Bioengineering
A “Plug-And-Display” Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

A “Plug-And-Display” Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

DOI:
10.3791/64213-v

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08:07 min

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July 25, 2022

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, , , , , ,
1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy,Third Military Medical University

Chapters

  • 00:05Introduction
  • 00:44OMV-SC Preparation
  • 03:02RBD-ST Preparation
  • 05:22OMV-RBD Bioconjugation and Purification
  • 06:39Results: OMV-RBD Verification, Particle Size Distribution, and Visualization by TEM
  • 07:42Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes the bioengineering of outer membrane vesicles to be a "Plug-and-Display" vaccine platform, including production, purification, bioconjugation, and characterization.

Tags

Keywords: Nanoparticle VaccineOuter Membrane VesiclesSARS-CoV-2 Receptor-binding DomainAntigen DisplayCytolysin A SpycatcherOMV Spycatcher ProductionOMV Spycatcher PurificationRBD Spytag TransfectionEukaryotic Expression System

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