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Quantitative Bestimmung der de novo Fettsäuresynthese im braunen Fettgewebe mittels Deuteriumoxid
JoVE Journal
Biology
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JoVE Journal Biology
Quantitative Determination of De Novo Fatty Acid Synthesis in Brown Adipose Tissue Using Deuterium Oxide
DOI:

07:34 min

May 12, 2023

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Chapters

  • 00:04Introduction
  • 00:48Lipid Extraction from Adipose Tissue
  • 02:31Preparation of Fatty Acid Methyl Esters (FAMEs) and GCMS Analysis
  • 04:08Deuterium Acetone Exchange of Plasma Samples to Determine Body Water Enrichment
  • 05:04Results: Plasma Body Water Enrichment from D2O, and Brown Adipose Tissue Total Fatty Acid Enrichment and Synthesis after 3 Days of D2O Administration
  • 06:34Conclusion

Summary

Automatic Translation

In dieser Arbeit stellen wir eine kostengünstige quantitative Methode vor, die Deuteriumoxid- und Gaschromatographie-Massenspektrometrie (GCMS) zur Analyse der Gesamtfettsäure-De-novo-Lipogenese in braunem Fettgewebe in vivo verwendet.

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