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Organotypiske kulturer af voksen human cortex som ex vivo-model for transplantation og validering af humane stamceller
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Chapters
- 00:05Introduction
- 01:14Tissue Collection, Maintenance, Cutting, and Plating
- 03:25Transplantation of the GFP-lt-NES Cells into Organotypic Human Adult Cortical (hACtx) Slices
- 04:57Results: Characterization and Electrophysiological Properties of hACtx Neurons
- 06:49Conclusion
Denne protokol beskriver langsigtede organotypiske kulturer af voksen human cortex kombineret med ex vivo intrakortikal transplantation af inducerede pluripotente stamcelleafledte kortikale forfædre, som præsenterer en ny metode til yderligere test af stamcellebaserede terapier for humane neurodegenerative lidelser.
