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Chapters
- 00:04Introduction
- 00:43Sample Extraction
- 01:47Sample Processing for Analysis of Hematopoietic Cell Populations in Total Bone Marrow
- 03:19Sample Processing for the Analysis of Stromal Cell Populations in Total Bone Marrow
- 05:13Sample Processing for the Analysis of Hematopoietic Cell Populations in Crushed and Flushed Bone Marrow
- 06:28Results: Phenotypic Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells by Flow Cytometry
- 07:40Conclusion
Qui, descriviamo un semplice protocollo per l'isolamento e la colorazione delle cellule del midollo osseo murino al fenotipo delle cellule staminali emopoietiche e progenitrici insieme alle cellule staminali endoteliali e mesenchimali di nicchia di supporto. È incluso anche un metodo per arricchire le cellule situate nelle aree endostali e centrali del midollo osseo.
