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Brug af In Vivo-billeddannelse til screening for morfogenesefænotyper i Candida albicans mutante stammer under aktiv infektion i en pattedyrvært
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Use of In Vivo Imaging to Screen for Morphogenesis Phenotypes in Candida albicans Mutant Strains During Active Infection in a Mammalian Host

Brug af In Vivo-billeddannelse til screening for morfogenesefænotyper i Candida albicans mutante stammer under aktiv infektion i en pattedyrvært

DOI:
10.3791/64258-v

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09:24 min

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October 12, 2022

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, ,
1Department of Pediatrics, Carver College of Medicine,University of Iowa, 2Department of Microbiology and Immunology, Carver College of Medicine,University of Iowa

Chapters

  • 00:04Introduction
  • 00:52Candida albicans Strains and Animal Preparation
  • 02:08Hair Removal and Inoculation
  • 04:06Quantify In Vitro Morphogenesis for Comparison to In Vivo Results
  • 04:42Preparation for In Vivo Imaging
  • 06:37Imaging
  • 07:38Manual Two‐Dimensional Analysis: Frequency of Filamentation
  • 07:59Results: Analyzing In Vitro and In Vivo Morphology of the Mutant Strains
  • 08:51Conclusion

Summary

Automatic Translation

Automatic Translation

Dette manuskript beskriver en metode til screening af moderat størrelse Candida albicans mutantbiblioteker for morfogenesefænotyper under aktiv infektion i en pattedyrsvært ved hjælp af ikke-invasiv konfokal mikroskopi.

Tags

Keywords: In Vivo ImagingCandida AlbicansMorphogenesisMammalian HostIntradermal InjectionChlorophyll-free ChowYPDDPBSHemocytometerDepilatory Cream70% EthanolInsulin Syringe

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