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Preparation of a Non-Cardiomyocyte Cell Suspension for Single-Cell RNA Sequencing from a Post-Myocardial Infarction Adult Mouse Heart
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Preparation of a Non-Cardiomyocyte Cell Suspension for Single-Cell RNA Sequencing from a Post-Myocardial Infarction Adult Mouse Heart

Preparation of a Non-Cardiomyocyte Cell Suspension for Single-Cell RNA Sequencing from a Post-Myocardial Infarction Adult Mouse Heart

DOI:
10.3791/64290-v

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05:54 min

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February 03, 2023

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, , , ,
1Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province, 2Department of Cardiology, Sir Run Run Shaw Hospital, School of Medicine,Zhejiang University Hangzhou

Chapters

  • 00:04Introduction
  • 01:02Heart Dissection
  • 02:07Preparation of Cardiac Cell Suspension
  • 04:24Results: Physical and Molecular Attributes of Cardiac Cells
  • 05:07Conclusion

Summary

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Automatic Translation

Here, we describe a protocol to isolate a sufficient amount of single non-cardiomyocytes with high viability from a post-myocardial infarction (MI) mouse heart. This can be used for subsequent single-cell sequencing, flow cytometry analysis, and primary cell culture.

Tags

Single-cell RNA SequencingNon-cardiomyocyte Cell SuspensionPost-myocardial InfarctionAdult Mouse HeartCell ViabilityIsolation EfficiencyLow-costCardiac PathophysiologyImmune CellsMyocardial InfarctionCardiac Dissociation BufferCell StrainerRBC Lysis Buffer

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