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Immunology and Infection
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A Minimally Invasive, Accurate, and Efficient Technique for Intrathymic Injection in Mice
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Minimally Invasive, Accurate, and Efficient Technique for Intrathymic Injection in Mice

A Minimally Invasive, Accurate, and Efficient Technique for Intrathymic Injection in Mice

DOI:
10.3791/64309-v

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07:17 min

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August 23, 2022

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, , ,
1Department of Radiology,Hackensack University Medical Center, 2Center for Discovery and Innovation,Hackensack Meridian Health, 3Department of Pediatrics,Hackensack University Medical Center, 4Department of Oncology,Georgetown University

Chapters

  • 00:04Introduction
  • 00:59Animal Preparation
  • 01:47Preparation of the Ultrasound Machine and Sterile Field
  • 03:14Imaging and Locating the Thymus
  • 03:55Injection of the Thymus
  • 05:45Results: An Ultrasound‐Guided Free‐Hand Intrathymic Injection in Mice
  • 06:47Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes an interventional radiology procedure established for intrathymic injection in mice to avoid the risk of open surgery and improve the accuracy of blind percutaneous injections.

Tags

Keywords: Minimally InvasiveAccurateEfficientIntrathymic InjectionMiceNon-surgicalUltrasound-guidedThymusAtrophyImmunosuppressionAnesthesiaDisinfectionUltrasound SettingsSterile Technique

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