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Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis
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1Department of Clinical Immunology,Wroclaw Medical University, 2Department of Medical Physiology, Chair of Biomedical Sciences, Institute of Physiotherapy, Faculty of Health Sciences,Jagiellonian University Medical College, 3Chair of Biomedical Sciences, Faculty of Health Sciences,Jagiellonian University Medical College
Chapters
- 00:04Introduction
- 00:59Marking Mice for Identification, and Induction of Contact Hypersensitivity (CHS)
- 02:19Ear Biopsies for Ear Weight and Myeloperoxidase (MPO) Assay
- 03:48Vascular Permeability Test
- 04:43Serum Collection and Anti-TNP Immunoglobulin (IgG1) Antibody Measurement
- 05:16Adoptive Transfer of the CHS-Effector Cells
- 06:41Results: Study of the Contact Hypersensitivity Reaction in Mice
- 07:42Conclusion
Contact hypersensitivity (CHS) is a murine experimental model of allergic contact dermatitis (ACD). CHS is based on sensitization with reactive hapten by painting the shaved skin of the chest and abdomen, with a subsequent ear skin challenge with a diluted hapten, causing a swelling reaction that is assessed in various ways.
