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Rat Burn Model to Study Full-Thickness Cutaneous Thermal Burn and Infection
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1UNC Eshelman School of Pharmacy,University of North Carolina at Chapel Hill, 2Department of Biochemistry & Pharmacology, School of Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences,The University of Melbourne, 3Department of Microbiology & Immunology,University of North Carolina School of Medicine, 4Department of Surgery,University of North Carolina at Chapel Hill, 5Curriculum in Toxicology and Environmental Medicine,University of North Carolina at Chapel Hill, 6Department of Microbiology, Monash Biomedicine Discovery Institute,Monash University
Chapters
- 00:05Introduction
- 00:57Preparing Rats for the Burn Injury
- 01:54Inducing the Burn Injury in Rats
- 03:20Preparation of Bacterial Inoculum and Infection
- 04:26Evaluation of the Burn Injury and Postprocessing of Rat Samples and Bacterial Enumeration
- 05:37Results: A Simple and Reproducible Rat Burn Infection Model
- 07:59Conclusion
A model mimicking the clinical scenario of burn injury and infection is necessary for furthering burn research. The present protocol demonstrates a simple and reproducible rat burn infection model comparable to that in humans. This facilitates the study of burn and infections following burn for developing new topical antibiotic treatments.
