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Journal
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Immunology and Infection
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噬菌体介导的莱姆病螺旋体伯
氏疏螺旋
体的遗传操作
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete
Borrelia burgdorferi
Please note that all translations are automatically generated.
Click here for the English version.
噬菌体介导的莱姆病螺旋体伯
氏疏螺旋
体的遗传操作
DOI:
10.3791/64408-v
•
09:01 min
•
September 28, 2022
•
Christian H. Eggers
1
Department of Biomedical Sciences
,
Quinnipiac University
Chapters
00:04
Introduction
00:40
Preparation of
Borrelia burgdorferi
Culture for the Production of φBB-1 and Induction of Borrelia burgdorferi Phage φBB-1
02:31
Polyethylene Glycol Precipitation to Recover Phage for Use in Transduction Assay
05:34
Transduction Assay Following Polyethylene Glycol Precipitation of φBB-1 and Selection of Transductants
06:51
Results: Transducing Heterologous DNA Between Different Strains of the Lyme Disease Spirochete Using
Borrelia burgdorferi
Bacteriophage
08:12
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
噬菌体在细菌细胞之间移动DNA的能力使其成为细菌宿主遗传操作的有效工具。这里介绍的是一种诱导、恢复和使用 φBB-1(
伯氏疏
螺旋体噬菌体)在莱姆病螺旋体的不同菌株之间转导异源 DNA 的方法。
Tags
Phage Transduction
Borrelia Burgdorferi
Lyme Disease
Molecular Biology
Genetic Manipulation
Phage Production
PEG Precipitation
Antibiotic Selection
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伯氏疏螺旋体(
Borrelia
)伯氏
疏螺旋
体
外
转录测定的基本成分
Read Article