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Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes

Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes

DOI:
10.3791/64439-v

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08:24 min

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November 11, 2022

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, ,
1Animal Sciences Research Center,University of Missouri, 2Universidad Nacional de Colombia, 3Advanced Light Microscopy Core,University of Missouri

Chapters

  • 00:05Introduction
  • 01:12Mouse Oocyte Collection
  • 02:53Oocyte Microinjection
  • 04:16Oocyte Maturation
  • 05:02Microscope Preparation for Ablation
  • 06:05Ablation of Metaphase Cytoplasmic Microtubule Organizing Centers (mcMTOCs)
  • 06:59Results: Depletion of Metaphase Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
  • 07:35Conclusion

Summary

Automatic Translation

Automatic Translation

An optimized protocol is presented that enables the depletion of cytoplasmic microtubule organizing centers in mouse oocytes during metaphase I using a near-infrared femtosecond laser.

Tags

Multi-photon Laser AblationCytoplasmic Microtubule Organizing CentersMouse OocytesSpindle PositioningMeiosisAneuploidyMTOCsPolar MTOCsMcMTOCsTargeted AblationLive CellPhototoxicityMechanical PerturbationCell Viability

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