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从小鼠子宫建立3D子宫内膜类器官

JoVE Journal
Biology

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JoVE Journal Biology

Establishing 3D Endometrial Organoids from the Mouse Uterus

Please note that all translations are automatically generated. Click here for the English version.

从小鼠子宫建立3D子宫内膜类器官

DOI:

10.3791/64448-v

•

06:24 min

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January 06, 2023

•

Suni Tang², Sydney E. Parks², Zian Liao², Dominique I. Cope², Sarah E. Blutt, Diana Monsivais²

¹Department of Pathology & Immunology,Baylor College of Medicine, ²Center for Drug Discovery,Baylor College of Medicine, ³Departments of Molecular Virology and Microbiology and Medicine,Baylor College of Medicine

Chapters

00:05Introduction
00:40Isolation of Uterine Epithelium from Mice Using Enzymatic and Mechanical Methods
03:18Encapsulation of Uterine Epithelium into Gel Matrix to Establish Organoids
04:19Results: Gene Expression and Histological Analyses of Mouse Endometrial Epithelial Organoids
05:32Conclusion

Summary

Automatic Translation

该协议描述了建立小鼠子宫内膜上皮类器官以进行基因表达和组织学分析的方法。

Tags

Keywords: 3D Endometrial Organoids Mouse Uterus Uterine Epithelium Enzymatic Dissociation Mechanical Dissociation Cell Culture Organoid Establishment Cell Pellet Collagenase DNA Solution

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