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Chapters
- 00:04Introduction
- 00:38Fabrication of the Two-Piece Channel and Modular Platform Base
- 04:28Injecting the COL1 Solution into the Microchannel and Removing the Cover for Cell Culture Applications
- 06:16Results: Confocal Reflectance Images of COL1 Fibers
- 06:45Conclusion
Denne protokol demonstrerer brugen af en mikrofluidisk kanal med skiftende geometri langs væskestrømningsretningen for at generere ekstensionel belastning (strækning) for at justere fibre i en 3D-kollagenhydrogel (<250 μm i tykkelse). Den resulterende justering strækker sig over flere millimeter og påvirkes af den ekstensionelle belastningshastighed.
