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从羊膜中提取Lumican及其储存温度的测定
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JoVE Journal Medicine
Lumican Extraction from Amniotic Membrane and Determination of its Storage Temperature
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从羊膜中提取Lumican及其储存温度的测定

DOI:
10.3791/64460-v

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04:21 min

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October 14, 2022

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, , ,
1Instituto de Oftalmologia Fundacion Conde de Valenciana IAP, 2Biochemistry Department, Medicine Faculty,Universidad Nacional Autónoma de Mexico

Chapters

  • 00:05Introduction
  • 00:37Preparation of the Amniotic Membrane Extract
  • 02:48Results: Determination of Storage Temperature for Amniotic Membrane Extract
  • 03:45Conclusion

Summary

Automatic Translation

Automatic Translation

本协议描述了从羊膜（AM）中提取lumican及其储存条件作为AM提取物（AME）在-20°C，4°C和室温（RT）下6，12，20和32天以量化其蛋白质和lumican浓度。

Tags

LumicanAmniotic MembraneProtein ExtractionBalanced Salt SolutionDispase IIDe-epithelializationLiquid NitrogenProtease InhibitorCentrifugationStorage Temperature

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