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Collection of Alfalfa Root Exudates to Study the Impact of Di(2-ethylhexyl) Phthalate on Metabolite Production
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JoVE Journal Environment
Collection of Alfalfa Root Exudates to Study the Impact of Di(2-ethylhexyl) Phthalate on Metabolite Production

Collection of Alfalfa Root Exudates to Study the Impact of Di(2-ethylhexyl) Phthalate on Metabolite Production

DOI:
10.3791/64470-v

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06:46 min

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June 02, 2023

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, , ,
1Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science,Chinese Academy of Sciences, 2College of Resources and Environment,University of Chinese Academy of Sciences

Chapters

  • 00:04Introduction
  • 00:37Hydroponic Culture Experiment
  • 02:04Collection, Extraction, and Metabolomic Analysis of Root Exudates
  • 04:40Results: Collection, Extraction, and Analysis of Alfalfa Root Exudates
  • 06:00Conclusion

Summary

Automatic Translation

Automatic Translation

The secretion of root exudates is usually an external detoxification strategy for plants under stress conditions. This protocol describes how to assess the impact of xenobiotics on alfalfa via nontargeted metabolomic analysis.

Tags

Keywords: AlfalfaRoot ExudatesDi(2-ethylhexyl) PhthalateDEHPXenobioticsMetabolomicsMedicago SativaSeed SterilizationGerminationRoot Exudate CollectionMetabolite ProfilingExtractionCentrifugation

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