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1Microscopy Core Facility, Technological Research and Scientific Area (ACTI),University of Murcia, 2Confocal Microscopy and Cellular Imaging Unit, Genetic and Molecular Medicine Department, Pediatric Institute for Rare Diseases,Hospital Sant Joan de Déu, 3Research Institute Sant Joan de Déu, Esplugues de Llobregat, Spain 4Algology Laboratory, Biology Faculty,University of Murcia, 4Algology Laboratory, Biology Faculty,University of Murcia
Chapters
- 00:05Introduction
- 00:47Sample Preparation for Reproducing Monochromatic Light Effect
- 01:50Autofluorescence Imaging
- 03:16Parameters to Evaluate Chroothece’s Physiology
- 04:21Results: Autofluorescence Imaging and Evaluation of Phycobiliprotein Changes in Red Algae Based on Spectral Analysis
- 05:08Conclusion
Denne protokol beskriver trin-for-trin autofluorescensbilleddannelse og evaluering af fykobiliproteinændringer i rødalger baseret på spektralanalyse. Dette er en etiketfri og ikke-destruktiv metode til evaluering af cellulær tilpasning til ekstreme levesteder, når kun knappe materialer er tilgængelige, og celler vokser langsomt eller slet ikke under laboratorieforhold.
