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Assessing the Putative Anticryptococcal Properties of Crude and Clarified Extracts from Mollusks
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Assessing the Putative Anticryptococcal Properties of Crude and Clarified Extracts from Mollusks

Assessing the Putative Anticryptococcal Properties of Crude and Clarified Extracts from Mollusks

DOI:
10.3791/64540-v

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09:18 min

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December 02, 2022

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, , ,
1Molecular and Cellular Biology Department,University of Guelph, 2School of Environmental Sciences,University of Guelph

Chapters

  • 00:05Introduction
  • 00:48Protein Extraction from Mollusks
  • 02:48Clarification of the Mollusk Extract
  • 03:55Inhibitory Activity Assay
  • 05:29Effect of Mollusk Extracts on Cryptococcus neoformans Growth
  • 06:52Results: Isolation of Proteins and Peptides from Mollusks with Potential Anti-Virulence Properties Against Cryptococcus neoformans
  • 08:14Conclusion

Summary

Automatic Translation

Automatic Translation

The human fungal pathogen Cryptococcus neoformans produces a variety of virulence factors (e.g., peptidases) to promote its survival within the host. Environmental niches represent a promising source of novel natural peptidase inhibitors. This protocol outlines the preparation of extracts from mollusks and the assessment of their effect on fungal virulence factor production.

Tags

MolluskAntifungalAnti-cryptococcalCompound IsolationNatural ProductsC. ChinensisCrude ExtractClarified ExtractHomogenizationCentrifugationFiltrationHeat Treatment

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