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Intra-Cardiac Injection of Human Prostate Cancer Cells to Create a Bone Metastasis Xenograft Mouse Model
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Cancer Research
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JoVE Journal Cancer Research
Intra-Cardiac Injection of Human Prostate Cancer Cells to Create a Bone Metastasis Xenograft Mouse Model

Intra-Cardiac Injection of Human Prostate Cancer Cells to Create a Bone Metastasis Xenograft Mouse Model

DOI:
10.3791/64589-v

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06:32 min

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November 04, 2022

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, , , , , , ,
1Longhua Hospital,Shanghai University of Traditional Chinese Medicine, 2Key Laboratory of Theory and Therapy of Muscles and Bones,Ministry of Education, 3Institute of Chinese Traditional Surgery, Longhua Hospital,Shanghai University of Traditional Chinese Medicine

Chapters

  • 00:04Introduction
  • 01:00Preparation of Prostate Cancer Cells for Injection
  • 02:25Surgery for Intra‐Cardiac Injection of the Human Prostate Cancer Cells
  • 05:03Results: Intra‐Cardiac Injection of Prostate Cancer Cells Induced Metastatic Lesions in Mice
  • 06:04Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for the intra-cardiac injection of human prostate cancer cells to generate a mouse model with bone metastasis lesions.

Tags

Intra-cardiac InjectionProstate Cancer CellsBone MetastasisXenograft Mouse ModelLuciferase-labeled PC3 CellsCell CultureTrypsinizationCell CountingCentrifugationCell SuspensionAnesthesiaSurgical ProcedureXiphoid ProcessJugular NotchIntercostal SpaceInjection Point

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