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Bestemmelse af haploider parringseffektivitet i Saccharomyces cerevisiae
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JoVE Journal Biology
Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae

Bestemmelse af haploider parringseffektivitet i Saccharomyces cerevisiae

DOI:
10.3791/64596-v

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05:39 min

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December 02, 2022

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, ,
1Department of Chemical Engineering,Indian Institute of Technology Bombay, 2Department of Ecology and Evolutionary Biology,University of Michigan, Ann Arbor

Chapters

  • 00:04Introduction
  • 00:46Patching of Haploids in the Mating Efficiency Grids
  • 01:55Mixing of Haploids and Mating
  • 02:55Plating of Mixed Cells on YPD Agar and Screening for Diploids using Auxotrophic Markers
  • 03:53Results: Quantification and Dynamics of Mating Efficiency in Saccharomyces cerevisiae (Yeast)
  • 05:03Conclusion

Summary

Automatic Translation

Automatic Translation

I dette arbejde beskrives en robust metode til kvantificering af parringseffektivitet i gæren Saccharomyces cerevisiae . Denne metode er især nyttig til kvantificering af præzygotiske barrierer i specieringsundersøgelser.

Tags

Yeast MatingHaploid Mating EfficiencySaccharomyces CerevisiaeMating AssayMating TypeCell GrowthOptical DensityCell SuspensionIncubationMating Grid

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