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Berigelse af voksne musedorsalrodganglier neuronkulturer ved immunpanorering
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1Neuroscience and Mental Health Institute,University of Alberta, 2Department of Medicine, Division of Neurology,University of Alberta, 3Department of Medical Microbiology and Immunology,University of Alberta, 4Department of Pharmacology,University of Alberta, 5Department of Anesthesiology and Pain Medicine,University of Alberta
Chapters
- 00:04Introduction
- 00:43Dissection, Trituration, and Immunopanning
- 07:54Results: DRG Culture Neuronal Enrichment by Immunopanning
- 08:31Conclusion
Dette papir beskriver en immunpanoreringsprotokol for voksne musedorsalrodsganglier. Ved at klæbe antistoffer til dyrkningsplader kan vi negativt vælge og fjerne ikke-neuronale celler. Vi viser, at kulturerne er beriget for neuroner ved hjælp af denne protokol, hvilket giver mulighed for en dybdegående undersøgelse af neuronale reaktioner på manipulation.
