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Testing Cancer Immunotherapeutics in a Humanized Mouse Model Bearing Human Tumors
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Cancer Research
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JoVE Journal Cancer Research
Testing Cancer Immunotherapeutics in a Humanized Mouse Model Bearing Human Tumors

Testing Cancer Immunotherapeutics in a Humanized Mouse Model Bearing Human Tumors

DOI:
10.3791/64606-v

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15:24 min

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December 16, 2022

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, , , , , , , , ,
1Department of Immunology and Microbiology, School of Medicine,University of Colorado Denver Anschutz Medical Campus, 2Division of Oncology, School of Medicine,University of Colorado Denver Anschutz Medical Campus, 3Department of Medical Oncology,Catalan Institute of Oncology (ICO-L’Hospitalet)

Chapters

  • 00:04Introduction
  • 01:06Generation of Human Immune System Mice
  • 04:59Testing Human Chimerism in Blood
  • 08:36Harvesting of Mouse Tissues and Tumors
  • 11:18Results: Evaluation of Chimerism, Tumor Growth Measurements, and Analysis of HIS Cells
  • 14:17Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol outlines the generation of human immune system (HIS) mice for immuno-oncology studies. Instructions and considerations in the use of this model for testing human immunotherapeutics on human tumors implanted in this model are presented with an emphasis on characterizing the response of the human immune system to the tumor.

Tags

Humanized Mouse ModelHuman TumorImmunotherapyCD34-positive CellsCord BloodCell ExpansionCell SeparationFlow CytometryPreclinical ModelImmuno-oncology

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