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Chapters
- 00:05Introduction
- 00:51Pre-ELISA Steps: Prerequisites and Preprocessing
- 01:42ELISA Steps: Capture Antibody Coating and Blocking
- 02:28ELISA Steps: Application of Antigen and Serum Samples
- 03:16ELISA Steps: Enzyme-Conjugated Antibody Addition and Detection
- 04:02Results: Validation of Non-Adsorbed and Adsorbed Mycoplasma pneumoniae Antiserum
- 05:19Conclusion
Tags
Antigen-Capture ELISAMycoplasma PneumoniaeSpecific DetectionCross-reaction DepletionAdsorption TechniqueHigh SpecificityReliable Screening TestHeterologous Bacterial AntigensPolyclonal Anti-Mycoplasma Pneumoniae IgGCapture AntibodyBlocking SolutionMycoplasma Pneumoniae Whole ProteinsHuman Serum Test SamplesReference Positive And Negative Serum Controls
