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AlDeSense在基于醛脱氢酶1A1活性的卵巢癌细胞分层中的应用

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Cancer Research

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JoVE Journal Cancer Research

Application of AlDeSense to Stratify Ovarian Cancer Cells Based on Aldehyde Dehydrogenase 1A1 Activity

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AlDeSense在基于醛脱氢酶1A1活性的卵巢癌细胞分层中的应用

DOI:

10.3791/64713-v

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09:59 min

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March 31, 2023

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Michael C. Lee*^1,3,4, Sarah H. Gardner*^2,3,4, Rodrigo Tapia Hernandez^3,4, Jefferson Chan^2,3,4

¹Department of Chemistry,University of Illinois Urbana-Champaign, ²Department of Biochemistry,University of Illinois Urbana-Champaign, ³Beckman Institute for Advanced Science and Technology,University of Illinois Urbana-Champaign, ⁴Cancer Center of Illinois,University of Illinois Urbana-Champaign

Chapters

00:04Introduction
00:38Fluorescence Microscopy Imaging of Cells with High ALDH1A1 Activity
03:03Application of Flow Cytometry to Identify Cells with High ALDH1A1 Activity
07:20Results: Total ALDH1A1 Activity and Identifying the Population of ALDH1A1+ Cells
09:33Conclusion

Summary

Automatic Translation

测量活细胞中 ALDH1A1 活性的方法在癌症研究中至关重要，因为它是干性的生物标志物。在这项研究中，我们采用了亚型选择性荧光探针来确定一组五种卵巢癌细胞系中 ALDH1A1 活性的相对水平。

Tags

Keywords: ALDH1A1 Stem Cell Biomarker AlDeSense Flow Cytometry Live Cell Imaging Fluorogenic Probe Confocal Microscopy Cell Culture Cell Counting Cell Detachment Trypsin Image Analysis

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