Journal
/
Cancer Research
/
Non-Invasive Ultrasound Assessment of Endometrial Cancer Progression in Pax8-Directed Deletion of the Tumor Suppressors Arid1a and Pten in Mice
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Non-Invasive Ultrasound Assessment of Endometrial Cancer Progression in Pax8-Directed Deletion of the Tumor Suppressors Arid1a and Pten in Mice

Non-Invasive Ultrasound Assessment of Endometrial Cancer Progression in Pax8-Directed Deletion of the Tumor Suppressors Arid1a and Pten in Mice

DOI:
10.3791/64732-v

•

07:44 min

•

February 17, 2023

•

, , , , , , , , ,
1Department of Molecular and Comparative Pathobiology,Johns Hopkins University School of Medicine, 2Department of Obstetrics and Gynecology,Johns Hopkins University School of Medicine, 3Texas Tech University School of Veterinary Medicine, 4Department of Pathology,Johns Hopkins University School of Medicine, 5Department of Surgery,Johns Hopkins University School of Medicine, 6Department of Coloproctological Surgery,Juntendo University School of Medicine, 7Department of Human Pathology,Juntendo University School of Medicine, 8Sidney Kimmel Comprehensive Cancer Center,Johns Hopkins University

Chapters

  • 00:04Introduction
  • 00:55Setting‐Up of Ultrasound Machine
  • 01:43Intraperitoneal Injection of Fluid to Increase the Contrast Between Organs
  • 02:37Ultrasound Imaging from a Dorsal Approach
  • 04:32Collecting Images from a Ventral Approach
  • 05:33Results: The Correlation Between Tissues Observed by Ultrasound and Histopathology
  • 06:49Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a method for monitoring the progression of morphological changes over time in the uterus in an inducible mouse model of endometrial cancer using ultrasound imaging with correlation to gross and histological changes.

Tags

Keywords: Non-invasiveUltrasoundEndometrial CancerProgressionPax8Arid1aPtenMouse ModelLongitudinal StudyImagingUterusOvaryTransducerB-modePeritoneal CavityAnesthesiaHeating PadUltrasound Gel

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/64732?article_type=v

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics
check_url/64732?article_type=v

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment
check_url/64732?article_type=v

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models
check_url/64732?article_type=v

Utilization of Ultrasound Guided Tissue-directed Cellular Implantation for the Establishment of Biologically Relevant Metastatic Tumor Xenografts
check_url/64732?article_type=v

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
check_url/64732?article_type=v

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
check_url/64732?article_type=v

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment
check_url/64732?article_type=v

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
check_url/64732?article_type=v

Detection of Lung Tumor Progression in Mice by Ultrasound Imaging
check_url/64732?article_type=v

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice

Read Article