Journal
/
Genetics
/
Mouse In Vivo Placental Targeted CRISPR Manipulation
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Mouse In Vivo Placental Targeted CRISPR Manipulation

Mouse In Vivo Placental Targeted CRISPR Manipulation

DOI:
10.3791/64760-v

•

07:39 min

•

April 14, 2023

•

, ,
1Interdisciplinary Graduate Program in Genetics,University of Iowa, 2Department of Psychiatry, Carver College of Medicine,University of Iowa, 3Iowa Neuroscience Institute, Carver College of Medicine,University of Iowa, 4Hawk-IDDRC,University of Iowa

Chapters

  • 00:04Introduction
  • 00:48Surgery Preparation and Uterine Laparotomy
  • 02:34Placental Injection and Electroporation
  • 04:55Results: A Targeted In Vivo CRISPR Manipulation of the Mouse Placenta
  • 07:01Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a time-specific method to effectively manipulate critical developmental pathways in the mouse placenta in vivo. This is performed through the injection and electroporation of CRISPR plasmids into the placentas of pregnant dams on embryonic day 12.5.

Tags

MousePlacentaCRISPRGenetic ManipulationIn VivoTargetedMid-late PregnancyOverexpressionAnesthesiaLaparotomyUterine HornPlacental InjectionElectroporation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast

Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

Manipulation of Ploidy in Caenorhabditis elegans

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Manipulation of Gene Function in Mexican Cavefish

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Read Article