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Developmental Biology
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研究卵巢切除小鼠模型中子宫对妊娠建立的贡献的方法
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Developmental Biology
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JoVE Journal
Developmental Biology
Methods for Studying Uterine Contributions to Pregnancy Establishment in an Ovariectomized Mouse Model
Please note that all translations are automatically generated.
Click here for the English version.
研究卵巢切除小鼠模型中子宫对妊娠建立的贡献的方法
DOI:
10.3791/64763-v
•
06:49 min
•
April 07, 2023
•
Meaghan J. Griffiths*
1,2
,
Jordan N. Higgins*
1
,
Fiona L. Cousins
,
Lauren R. Alesi
,
Amy L. Winship*
1
,
Karla J. Hutt*
1
1
Biomedicine Discovery Institute, Department of Anatomy and Developmental Biology
,
Monash University
,
2
Gynaecology Research Centre, Royal Women’s Hospital and Department of Obstetrics and Gynaecology
,
University of Melbourne
,
3
The Ritchie Centre, Hudson Institute for Medical Research and Department of Obstetrics and Gynaecology
,
Monash University
Chapters
00:04
Introduction
00:54
Murine Ovariectomy
03:09
Slow-Release Hormone Pellet Preparation
04:35
Artificial Decidualization
05:51
Results: A Well-Characterized Model of Murine Uterine Artificial Decidualization
06:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
怀孕的建立是一个动态的过程,涉及复杂的胚胎和子宫串扰。母体子宫环境对这些过程的确切贡献仍然是一个活跃的研究领域。在这里,提供了详细的协议来帮助设计
体内
动物模型来解决这些研究问题。
Tags
Ovariectomy
Uterine Contribution
Pregnancy Establishment
HPG Axis
Mouse Model
Surgical Techniques
Anesthesia
Analgesia
Sutures
Incision
Ovarian Fat Pad
Uterine Horn
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