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Monitoring Changes in Human Umbilical Vein Endothelial Cells upon Viral Infection Using Impedance-Based Real-Time Cell Analysis
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Monitoring Changes in Human Umbilical Vein Endothelial Cells upon Viral Infection Using Impedance-Based Real-Time Cell Analysis

Monitoring Changes in Human Umbilical Vein Endothelial Cells upon Viral Infection Using Impedance-Based Real-Time Cell Analysis

DOI:
10.3791/64887-v

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07:56 min

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May 05, 2023

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, , ,
1Tropical Infectious Diseases Research and Education Centre (TIDREC), Higher Institution Center of Excellence (HICoE),Universiti Malaya, 2Department of Medical Microbiology, Faculty of Medicine,Universiti Malaya

Chapters

  • 00:00Introduction
  • 00:36Setup of RTCA Experiment
  • 04:03Viral Infection in HUVECs
  • 06:06Results: Normalized Cell Index Versus Time Plot of Zika Virus Infection in Human HUVECs
  • 07:38Conclusion

Summary

Automatic Translation

Automatic Translation

The current study describes a protocol to monitor the changes in human umbilical vein endothelial cells (HUVECs) during viral infection using a real-time cell analysis (RTCA) system.

Tags

Keywords: HUVECImpedance-based Real-time Cell AnalysisVascular IntegrityViral InfectionTransient EffectCell MorphologyNon-invasiveRTCA Software96-well PlateCollagen Type IExtracellular MatrixCell IndexVerification Run

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