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氯化铁诱导的动脉血栓形成和用于3D电子显微镜分析的样本采集
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Medicine
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JoVE Journal
Medicine
Ferric Chloride-Induced Arterial Thrombosis and Sample Collection for 3D Electron Microscopy Analysis
Please note that all translations are automatically generated.
Click here for the English version.
氯化铁诱导的动脉血栓形成和用于3D电子显微镜分析的样本采集
DOI:
10.3791/64985-v
•
03:57 min
•
March 17, 2023
•
Smita Joshi*
1
,
Alexis N. Smith
,
Kanakanagavalli Shravani Prakhya
,
Hammodah R. Alfar
,
Joshua Lykins
,
Ming Zhang
,
Irina Pokrovskaya
,
Maria Aronova
,
Richard D. Leapman
,
Brian Storrie
,
Sidney W. Whiteheart*
1
1
Department of Molecular and Cellular Biochemistry
,
University of Kentucky
,
2
Department of Physiology and Cell Biology
,
University of Arkansas for Medical Sciences
,
3
Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering
,
National Institutes of Health
Chapters
00:05
Introduction
00:48
Sample Preparation for Serial Block Face Scanning Electron Microscopy Studies Post Ferric Chloride‐Induced Injury
02:47
Results: Ferric Chloride‐Mediated Injury to Induce Arterial Thrombosis
03:29
Conclusion
Summary
Automatic Translation
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Automatic Translation
本协议描述了如何使用FeCl
3
介导的损伤来诱导动脉血栓形成,以及如何在血栓形成的各个阶段收集和制备动脉损伤样品以进行电子显微镜分析。
Tags
Ferric Chloride-Induced Arterial Thrombosis
3D Electron Microscopy Analysis
Platelets
Hemostatic System
Thrombosis
Carotid Artery
Iron Chloride Injury
Flow Measurements
Fixative
Electron Microscopy
Blood Flow
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