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Generering af genetisk modificerede Plasmodium berghei sporozoitter
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Chapters
- 00:04Introduction
- 00:57Generation of Schizonts in Culture
- 02:25Transfection of Plasmodium Schizonts
- 04:58Selection of the Transfected Parasites
- 05:52Infection of Mosquitoes with the Transgenic Lines
- 07:15Collection of Sporozoites
- 08:53Results: Detecting the Lysis of Plasmodium and its Parasitophorous Vacuole in Host Hepatocytes
- 09:42Conclusion
Malaria overføres gennem podning af sporozoitstadiet af Plasmodium af inficerede myg. Transgen Plasmodium har gjort det muligt for os at forstå malariaens biologi bedre og har bidraget direkte til udviklingen af malariavacciner. Her beskriver vi en strømlinet metode til at generere transgene Plasmodium berghei sporozoitter.
