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Evaluering af autofaginiveauer i to forskellige bugspytkirtelcellemodeller ved hjælp af LC3-immunofluorescens
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JoVE Journal Biology
Evaluating Autophagy Levels in Two Different Pancreatic Cell Models Using LC3 Immunofluorescence

Evaluering af autofaginiveauer i to forskellige bugspytkirtelcellemodeller ved hjælp af LC3-immunofluorescens

DOI:
10.3791/65005-v

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08:07 min

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April 28, 2023

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, , , ,
1Instituto de Bioquimica y Medicina Molecular Prof Alberto Boveris (IBIMOL),Universidad de Buenos Aires, CONICET, 2Signalling Programme,The Babraham Institute

Chapters

  • 00:05Introduction
  • 00:49PANC-1 Cell Preparation and Gemcitabine Treatment
  • 01:56Fixation, Permeabilization, and Blocking the PANC-1 Cells
  • 02:32Antibody Incubation
  • 03:57DAPI Staining, Montage, and Visualization of the Cells
  • 04:51LC3 Dots Quantification
  • 06:13Results: Effect of LC3 Immunofluorescence in Pancreatic Cell Lines
  • 07:26Conclusion

Summary

Automatic Translation

Automatic Translation

Målet med denne protokol er at bestemme autofagiske niveauer i kræft i bugspytkirtlen og pancreas acinarceller gennem LC3 immunofluorescens og LC3 dot kvantificering.

Tags

Keywords: AutophagyLC3 ImmunofluorescencePancreatic CellsCell PreparationGemcitabine TreatmentCell Fixation And PermeabilizationPrimary Antibody IncubationFluorescent Imaging

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